1.0 OBJECTIVE

• To describe procedure for Microbial Count on Hands by using dab test method.

2.0 SCOPE

• It applies to the all staff and workers who are coming in direct contact with product during Manufacturing/processing.

3.0 RESPONSIBILITY

• Microbiologist

4.0 PROCEDURE :

• Prepare SCDA plate and keep in incubator for preincubation at 30°C to 35°C for 24 hours.
• Those people who directly come in contact to product, this people  first wash the hand with help of water and then disinfect the hand with 70% IPA.
• Take the impression of finger on plate of media.
• After pressing the finger keep the plate in incubator for incubation at 20°C to 25°C for 3 days for fungal count and then 30°C to 35°C for bacterial count for 2 days.
• Observe the colonies after incubation and record.
• FREQUENCY:
  • Once in month.
• LIMITS:
  • Alert limit              : 60 cfu/hand
  • Action limit           : 80 cfu/hand
  • Maximum limit     : 100 cfu/hand
• In case of failure of result, give the proper training to all worker and staff to disinfect hand using 70% IPA.